RNA-induced epigenetic silencing inhibits HIV-1 reactivation from latency.

ABSTRACT:

Background

Current antiretroviral therapy is effective in controlling HIV-1 infection. However, cessation of therapy is associated with rapid return of viremia from the viral reservoir. Eradicating the HIV-1 reservoir has proven difficult with the limited success of latency reactivation strategies and reflects the complexity of HIV-1 latency. Consequently, there is a growing need for alternate strategies. Here we explore a "block and lock" approach for enforcing latency to render the provirus unable to restart transcription despite exposure to reactivation stimuli. Reactivation of transcription from latent HIV-1 proviruses can be epigenetically blocked using promoter-targeted shRNAs to prevent productive infection. We aimed to determine if independent and combined expression of shR

SUBMITTER: Mendez C 

PROVIDER: S-EPMC6172763 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature