Growth factor independence 1 expression in myeloma cells enhances their growth, survival, and osteoclastogenesis.

ABSTRACT:

Background

In spite of major advances in treatment, multiple myeloma (MM) is currently an incurable malignancy due to the emergence of drug-resistant clones. We previously showed that MM cells upregulate the transcriptional repressor, growth factor independence 1 (Gfi1), in bone marrow stromal cells (BMSCs) that induces prolonged inhibition of osteoblast differentiation. However, the role of Gfi1 in MM cells is unknown.

Methods

Human primary CD138+ and BMSC were purified from normal donors and MM patients' bone marrow aspirates. Gfi1 knockdown and overexpressing cells were generated by lentiviral-mediated shRNA. Proliferation/apoptosis studies were done by flow cytometry, and protein levels were determined by Western blot and/or immunohistochemistry. An experimental MM mouse

SUBMITTER: Petrusca DN 

PROVIDER: S-EPMC6172782 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature