Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence.

ABSTRACT:

Objective

The aim of this study is to investigate the presence of human papillomavirus DNA and genotypes in breast cancer and normal breast tissue samples obtained from women from the northeast region of Brazil.

Method

One hundred three breast cancer samples and 95 normal breast samples, as the non-malignant controls, were studied. DNA extraction was verified by human beta-globin gene amplification, and polymerase chain reaction was conducted based on HPV L1-specific consensus primers MY09/MY11 and GP5+/GP6+, followed by nested multiplex polymerase chain reaction with type-specific primers for the E6/E7 consensus region.

Results

Human papillomavirus DNA was detected in 51 (49.5%) breast carcinoma samples and 15 (15.8%) normal breast samples (p<0.0001). Human papilloma

SUBMITTER: Cavalcante JR 

PROVIDER: S-EPMC6172977 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature