Production of Deuterated Cyanidin 3-O-Glucoside from Recombinant Escherichia coli.

ABSTRACT: Anthocyanins are plant secondary metabolites that, despite their chemical instability, have found many applications as natural food colorants. They are also known for their beneficial health effects because of their antioxidant and anticancer properties. More stable versions of these molecules, particularly at neutral pH conditions, are required to study the anthocyanin pharmacokinetic properties and obtain effective therapeutic results. In the present report, a cost-effective technique was developed to prepare the deuterated anthocyanin using recombinant Escherichia coli as a production host and deuterated glycerol and D₂O in the culture media. This approach resulted in the formation of endogenous deuterated uridine 5'-diphosphate-glucose that was further incorporated by

SUBMITTER: Gupta M 

PROVIDER: S-EPMC6173498 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature