Hapten Design and Antibody Generation for Immunoanalysis of Spirotetramat and Spirotetramat-enol.

ABSTRACT: Spirotetramat-a tetramic acid insecticide-is rapidly metabolized or degraded to give spirotetramat-enol; so, common residue definitions include the sum of both compounds. In the present study, two spirotetramat-functionalized derivatives (haptens) have been designed to generate immunoreagents to these molecules for rapid immunochemical analysis. Haptens have been synthesized with alternative linker tethering sites and, for the first time, high-affinity antibodies have been generated with different specificities to these active principles. Two sensitive assays have been developed using the same antibody in different formats, and by using linker-site heterologous haptens, the selectivity of the final immunoassay could be improved. A generic immunoassay with sensitivity similar to spirotetram

SUBMITTER: Cevallos-Cedeno RE 

PROVIDER: S-EPMC6173556 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature