Temporal differentiation of bovine airway epithelial cells grown at an air-liquid interface.

ABSTRACT: There is an urgent need to develop improved, physiologically-relevant in vitro models of airway epithelia with which to better understand the pathological processes associated with infection, allergies and toxicological insults of the respiratory tract of both humans and domesticated animals. In the present study, we have characterised the proliferation and differentiation of primary bovine bronchial epithelial cells (BBECs) grown at an air-liquid interface (ALI) at three-day intervals over a period of 42 days from the introduction of the ALI. The differentiated BBEC model was highly representative of the ex vivo epithelium from which the epithelial cells were derived; a columnar, pseudostratified epithelium that was highly reflective of native airway epithelium was formed which comprised

SUBMITTER: Cozens D 

PROVIDER: S-EPMC6173764 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature