CpG binding protein (CFP1) occupies open chromatin regions of active genes, including enhancers and non-CpG islands.

ABSTRACT:

Background

The mechanism by which protein complexes interact to regulate the deposition of post-translational modifications of histones remains poorly understood. This is particularly important at regulatory regions, such as CpG islands (CGIs), which are known to recruit Trithorax (TrxG) and Polycomb group proteins. The CxxC zinc finger protein 1 (CFP1, also known as CGBP) is a subunit of the TrxG SET1 protein complex, a major catalyst of trimethylation of H3K4 (H3K4me3).

Results

Here, we used ChIP followed by high-throughput sequencing (ChIP-seq) to analyse genomic occupancy of CFP1 in two human haematopoietic cell types. We demonstrate that CFP1 occupies CGIs associated with active transcription start sites (TSSs), and is mutually exclusive with H3K27 trimethylation (H3K27

SUBMITTER: van de Lagemaat LN 

PROVIDER: S-EPMC6173865 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature