Protein Modification by Endogenously Generated Lipid Electrophiles: Mitochondria as the Source and Target.

ABSTRACT: Determining the impact of lipid electrophile-mediated protein damage that occurs during oxidative stress requires a comprehensive analysis of electrophile targets adducted under pathophysiological conditions. Incorporation of ω-alkynyl linoleic acid into the phospholipids of macrophages prior to activation by Kdo₂-lipid A, followed by protein extraction, click chemistry, and streptavidin affinity capture, enabled a systems-level survey of proteins adducted by lipid electrophiles generated endogenously during the inflammatory response. Results revealed a dramatic enrichment for membrane and mitochondrial proteins as targets for adduction. A marked decrease in adduction in the presence of MitoTEMPO demonstrated a primary role for mitochondrial superoxide in electrophile generation

SUBMITTER: Beavers WN 

PROVIDER: S-EPMC6174696 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature