"One-Pot" Sample Processing Method for Proteome-Wide Analysis of Microbial Cells and Spores.

ABSTRACT:

Purpose

Bacterial endospores, the transmissible forms of pathogenic bacilli and clostridia, are heterogeneous multilayered structures composed of proteins. These proteins protect the spores against a variety of stresses, thus helping spore survival, and assist in germination, by interacting with the environment to form vegetative cells. Owing to the complexity, insolubility, and dynamic nature of spore proteins, it has been difficult to obtain their comprehensive protein profiles.

Experimental design

The intact spores of Bacillus subtilis, Bacillus cereus, and Peptoclostridium difficile and their vegetative counterparts were disrupted by bead beating in 6 m urea under reductive conditions. The heterogeneous mixture was then double digested with LysC and trypsin. Next, the pe

SUBMITTER: Swarge BN 

PROVIDER: S-EPMC6174930 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature