Automation of the in vitro micronucleus assay using the Imagestream^® imaging flow cytometer.

ABSTRACT: The in vitro micronucleus (MN) assay is a well-established test for evaluating genotoxicity and cytotoxicity. The use of manual microscopy to perform the assay can be laborious and often suffers from user subjectivity and interscorer variability. Automated methods including slide-scanning microscopy and conventional flow cytometry have been developed to eliminate scorer bias and improve throughput. However, these methods possess several limitations such as lack of cytoplasmic visualization using slide-scanning microscopy and the inability to visually confirm the legitimacy of MN or storage of image data for re-evaluation using flow cytometry. The ImageStream^X® MK II (ISX) imaging flow cytometer has been demonstrated to overcome all of these limitations. The ISX combines the spee

SUBMITTER: Rodrigues MA 

PROVIDER: S-EPMC6174940 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature