Cell line-based xenograft mouse model of paediatric glioma stem cells mirrors the clinical course of the patient.

ABSTRACT: The leading cause of cancer-related mortality among children is brain tumour, and glioblastoma multiforme (GBM) has the worst prognosis. New treatments are urgently needed, but with few cases and clinical trials in children, pre-clinical models such as patient-derived tumour xenografts (PDTX) are important. To generate these, tumour tissue is transplanted into mice, but this yields highly variable results and requires serial passaging in mice, which is time-consuming and expensive. We therefore aimed to establish a cell line-based orthotopic mouse model representative of the patient tumour. Glioma stem cell (GSC) lines derived from paediatric GBM were orthotopically transplanted into immunodeficient mice. Overall survival data were collected and histological analysis of the resulting neopl

SUBMITTER: Larsson S 

PROVIDER: S-EPMC6175027 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature