Visualization and application of amino acid retention coefficients obtained from modeling of peptide retention.

ABSTRACT: We introduce a method for data inspection in liquid separations of peptides using amino acid retention coefficients and their relative change across experiments. Our method allows for the direct comparison between actual experimental conditions, regardless of sample content and without the use of internal standards. The modeling uses linear regression of peptide retention time as a function of amino acid composition. We demonstrate the pH dependency of the model in a control experiment where the pH of the mobile phase was changed in controlled way. We introduce a score to identify the false discovery rate on peptide spectrum match level that corresponds to the set of most robust models, i.e. to maximize the shared agreement between experiments. We demonstrate the method utility in reversed

SUBMITTER: Mohammed Y 

PROVIDER: S-EPMC6175132 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature