Cationic Europium Complexes for Visualizing Fluctuations in Mitochondrial ATP Levels in Living Cells.

ABSTRACT: The ability to study cellular metabolism and enzymatic processes involving adenosine triphosphate (ATP) is impeded by the lack of imaging probes capable of signalling the concentration and distribution of intracellular ATP rapidly, with high sensitivity. We report here the first example of a luminescent lanthanide complex capable of visualizing changes in the concentration of ATP in the mitochondria of living cells. Four cationic europium(III) complexes [Eu.1-4]⁺ have been synthesized and their binding capabilities towards nucleoside polyphosphate anions examined in aqueous solution at physiological pH. Complexes [Eu.1]⁺ and [Eu.3]⁺ bearing hydrogen bond donor groups in the pendant arms showed excellent discrimination between ATP, ADP and monophosphate spec

SUBMITTER: Mailhot R 

PROVIDER: S-EPMC6175470 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature