Heat stress induced, ligand-independent MET and EGFR signalling in hepatocellular carcinoma.

ABSTRACT:

Purpose

The aims of the present study were 2-fold: first, to test the hypothesis that heat stress induces MET and EGFR signalling in hepatocellular carcinoma (HCC) cells and inhibition of this signalling decreases HCC clonogenic survival; and second, to identify signalling pathways associated with heat stress induced MET signalling.

Materials and methods

MET⁺ and EGFR⁺ HCC cells were pre-treated with inhibitors to MET, EGFR, PI3K/mTOR or vehicle and subjected to heat stress or control ± HGF or EGF growth factors and assessed by colony formation assay, Western blotting and/or quantitative mass spectrometry. IACUC approved partial laser thermal or sham ablation was performed on orthotopic N1S1 and AS30D HCC tumours and liver/tumour assessed for phospho-ME

SUBMITTER: Thompson SM 

PROVIDER: S-EPMC6175538 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature