Tagging of Weakly Expressed Toxoplasma gondii Calcium-Related Genes with High-Affinity Tags.

ABSTRACT: Calcium ions regulate a diversity of cellular functions in all eukaryotes. The cytosolic Ca²⁺ concentration is tightly regulated at the physiological cytosolic concentration of 50-100 nm. The Toxoplasma gondii genome predicts the presence of several genes encoding potential Ca²⁺ channels, pumps, and transporters. Many of these genes are weakly expressed and likely tightly regulated due to their potential impact to the physiology of the cell. Endogenous tagging has been widely used to localize proteins in T. gondii but low level of expression of many of them makes visualization of tags difficult and sometimes impossible. The use of high-performance tags for labeling proteins expressed at low level is ideal for investigating the localization of these gene products. We d

SUBMITTER: Hortua Triana MA 

PROVIDER: S-EPMC6175649 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature