A reference document on Permissible Limits for solvents and buffers during in vitro antimalarial screening.

ABSTRACT: Antimalarial drug discovery expands on targeted and phenotype-based screening of potential inhibitory molecules to ascertain overall efficacy, phenotypic characteristics and toxicity, prior to exploring pharmacological optimizations. Candidate inhibitors may have varying chemical properties, thereby requiring specific reconstitution conditions to ensure solubility, stability or bioavailability. Hence, a variety of solvents, buffers, detergents and stabilizers become part of antimalarial efficacy assays, all of which, above certain threshold could interfere with parasite viability, invasion or red blood cell properties leading to misinterpretation of the results. Despite their routine use across malaria research laboratories, there is no documentation on non-toxic range for common constitue

SUBMITTER: Naidu R 

PROVIDER: S-EPMC6175914 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature