Insights Into Arsenite and Arsenate Uptake Pathways Using a Whole Cell Biosensor.

ABSTRACT: Despite its high toxicity and widespread occurrence in many parts of the world, arsenic (As) concentrations in decentralized water supplies such as domestic wells remain often unquantified. One limitation to effective monitoring is the high cost and lack of portability of current arsenic speciation techniques. Here, we present an arsenic biosensor assay capable of quantifying and determining the bioavailable fraction of arsenic species at environmentally relevant concentrations. First, we found that inorganic phosphate, a buffering agent and nutrient commonly found in most bioassay exposure media, was in fact limiting As(V) uptake, possibly explaining the variability in As(V) detection reported so far. Second, we show that the nature of the carbon source used in the bioassay differentially

SUBMITTER: Pothier MP 

PROVIDER: S-EPMC6176005 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature