A Comparison of Techniques for Collecting Skin Microbiome Samples: Swabbing Versus Tape-Stripping.

ABSTRACT: The swabbing and tape-stripping methods have traditionally been used for collecting skin microbiome samples for skin bacterial analysis, although no reports have compared the outcome of these methods for collecting skin bacteria. Our purpose was to show the differences in microbial composition between samples collected using the swabbing and tape-stripping methods, by both the next generation sequencing and culture studies. The skin microbiome was collected by both methods, and the samples were processed for a sequence-based microbiome analysis and culture study. The next-generation sequencing results showed that skin bacteria collected using the tape-stripping method were comparable to those collected using the swabbing method. In the culture study, the tape-stripping method collected a g

SUBMITTER: Ogai K 

PROVIDER: S-EPMC6176111 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature