Characterization of Inducible Transcription and Translation-Competent HIV-1 Using the RNAscope ISH Technology at a Single-Cell Resolution.

ABSTRACT: Identifying the source and dynamics of persistent HIV-1 at single-cell resolution during cART is crucial for the design of strategies to eliminate the latent HIV-1 reservoir. An assay to measure latent HIV-1 that can distinguish inducible from defective proviruses with high precision is essential to evaluate the efficacy of HIV-1 cure efforts but is presently lacking. The primary aim of this study was therefore to identify transcription and translation competent latently infected cells through detection of biomolecules that are dependent on transcriptional activation of the provirus. We investigated the applicability of two commercially available assays; PrimeFlow^TM RNA Assay (RNAflow) and RNAscope^® ISH (RNAscope) for evaluation of the efficacy of latency reversal age

SUBMITTER: Zhang W 

PROVIDER: S-EPMC6176121 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature