Identification of cell populations necessary for leaf-to-leaf electrical signaling in a wounded plant.

ABSTRACT: The identity of the cell files necessary for the leaf-to-leaf transmission of wound signals plants has been debated for decades. In Arabidopsis, wounding initiates the glutamate receptor-like (GLR)-dependent propagation of membrane depolarizations that lead to defense gene activation. Using a vein extraction procedure we found pools of GLR-fusion proteins in endomembranes in phloem sieve elements and/or in xylem contact cells. Strikingly, only double mutants that eliminated GLRs from both of these spatially separated cell types strongly attenuated leaf-to-leaf electrical signaling. glr3.3 mutants were also compromised in their defense against herbivores. Since wounding is known to cause increases in cytosolic calcium, we monitored electrical signals and Ca²⁺ transi

SUBMITTER: Nguyen CT 

PROVIDER: S-EPMC6176584 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature