Repurposing type III polyketide synthase as a malonyl-CoA biosensor for metabolic engineering in bacteria.

ABSTRACT: Malonyl-CoA is an important central metabolite for the production of diverse valuable chemicals including natural products, but its intracellular availability is often limited due to the competition with essential cellular metabolism. Several malonyl-CoA biosensors have been developed for high-throughput screening of targets increasing the malonyl-CoA pool. However, they are limited for use only in Escherichia coli and Saccharomyces cerevisiae and require multiple signal transduction steps. Here we report development of a colorimetric malonyl-CoA biosensor applicable in three industrially important bacteria: E. coli, Pseudomonas putida, and Corynebacterium glutamicum RppA, a type III polyketide synthase producing red-colored flaviolin, was repurposed as a

SUBMITTER: Yang D 

PROVIDER: S-EPMC6176597 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature