Assimilation of formic acid and CO₂ by engineered Escherichia coli equipped with reconstructed one-carbon assimilation pathways.

ABSTRACT: Gaseous one-carbon (C1) compounds or formic acid (FA) converted from CO₂ can be an attractive raw material for bio-based chemicals. Here, we report the development of Escherichia coli strains assimilating FA and CO₂ through the reconstructed tetrahydrofolate (THF) cycle and reverse glycine cleavage (gcv) pathway. The Methylobacterium extorquens formate-THF ligase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase genes were expressed to allow FA assimilation. The gcv reaction was reversed by knocking out the repressor gene (gcvR) and overexpressing the gcvTHP genes. This engineered strain synthesized 96% and 86% of proteinogenic glycine and serine, respectively, from FA and CO₂ in a glucose-containing medium. Native se

SUBMITTER: Bang J 

PROVIDER: S-EPMC6176599 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature