An orthotopic glioblastoma animal model suitable for high-throughput screenings.

ABSTRACT:

Background

Glioblastoma (GBM) is an aggressive form of brain cancer with poor prognosis. Although murine animal models have given valuable insights into the GBM disease biology, they cannot be used in high-throughput screens to identify and profile novel therapies. The only vertebrate model suitable for large-scale screens, the zebrafish, has proven to faithfully recapitulate biology and pathology of human malignancies, and clinically relevant orthotopic zebrafish models have been developed. However, currently available GBM orthotopic zebrafish models do not support high-throughput drug discovery screens.

Methods

We transplanted both GBM cell lines as well as patient-derived material into zebrafish blastulas. We followed the behavior of the transplants with time-lapse micros

SUBMITTER: Pudelko L 

PROVIDER: S-EPMC6176805 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature