Discovery and Validation of Methylation Biomarkers for Ulcerative Colitis Associated Neoplasia.

ABSTRACT:

Background and aims

Ulcerative colitis (UC) is associated with a higher background risk of dysplasia and/or neoplasia due to chronic inflammation. There exist few biomarkers for identification of patients with dysplasia, and targeted biopsies in this group of patients are inaccurate in reliably identifying dysplasia. We aimed to examine the epigenome of UC dysplasia and to identify and validate potential biomarkers.

Methods

Colonic samples from patients with UC-associated dysplasia or neoplasia underwent epigenome-wide analysis on the Illumina 450K methylation array. Markers were validated by bisulphite pyrosequencing on a secondary validation cohort and accuracy calculated using logistic regression and receiver-operator curves.

Results

Twelve samples from 4 patients

SUBMITTER: Beggs AD 

PROVIDER: S-EPMC6176894 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature