VEGF-induced endothelial cell migration requires urokinase receptor (uPAR)-dependent integrin redistribution.

ABSTRACT:

Aims

Vascular endothelial growth factor (VEGF)-initiated angiogenesis requires coordinated proteolytic degradation of extracellular matrix provided by the urokinase plasminogen activator/urokinase receptor (uPA/uPAR) system and regulation of cell migration provided by integrin-matrix interaction. In this study, we investigated the mechanisms underlying the uPAR-dependent modulation of VEGF-induced endothelial migration.

Methods and results

We used flow cytometry to quantify integrins at the cell surface. Stimulation of human and murine endothelial cells with VEGF resulted in internalization of α5β1-integrins. Micropatterning and immunocytochemistry revealed co-clustering of uPAR and α5β1-integrins and retrieval via clathrin-coated vesicles. It was also contingent on receptor

SUBMITTER: Alexander RA 

PROVIDER: S-EPMC6176909 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature