A Langendorff-like system to quantify cardiac pump function in adult zebrafish.

ABSTRACT: Zebrafish are increasingly used as a vertebrate model to study human cardiovascular disorders. Although heart structure and function are readily visualized in zebrafish embryos because of their optical transparency, the lack of effective tools for evaluating the hearts of older, nontransparent fish has been a major limiting factor. The recent development of high-frequency echocardiography has been an important advance for in vivo cardiac assessment, but it necessitates anesthesia and has limited ability to study acute interventions. We report the development of an alternative experimental ex vivo technique for quantifying heart size and function that resembles the Langendorff heart preparations that have been widely used in mammalian models. Dissected adult zebrafish hearts were perfused w

SUBMITTER: Zhang H 

PROVIDER: S-EPMC6177000 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature