IgM cleavage by Streptococcus suis reduces IgM bound to the bacterial surface and is a novel complement evasion mechanism.

ABSTRACT: Streptococcus suis (S. suis) causes meningitis, arthritis and endocarditis in piglets. The aim of this study was to characterize the IgM degrading enzyme of S. suis (Ide_Ssuis) and to investigate the role of IgM cleavage in evasion of the classical complement pathway and pathogenesis. Targeted mutagenesis of a cysteine in the putative active center of Ide_Ssuis abrogated IgM cleavage completely. In contrast to wt rIde_Ssuis, point mutated rIde_Ssuis_C195S did not reduce complement-mediated hemolysis indicating that complement inhibition by rIde_Ssuis depends on the IgM proteolytic activity. A S. suis mutant expressing Ide_Ssuis_C195S did not reduce IgM labeling, whereas the wt and complemented mutant showed less IgM F(ab')2 and Ig

SUBMITTER: Rungelrath V 

PROVIDER: S-EPMC6177247 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature