Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells.

ABSTRACT: Optogenetic switches are emerging molecular tools for studying cellular processes as they offer higher spatiotemporal and quantitative precision than classical, chemical-based switches. Light-controllable gene expression systems designed to upregulate protein expression levels meanwhile show performances superior to their chemical-based counterparts. However, systems to reduce protein levels with similar efficiency are lagging behind. Here, we present a novel two-component, blue light-responsive optogenetic OFF switch ('Blue-OFF'), which enables a rapid and quantitative down-regulation of a protein upon illumination. Blue-OFF combines the first light responsive repressor KRAB-EL222 with the protein degradation module B-LID (blue light-inducible degradation domain) to simultaneously control

SUBMITTER: Baaske J 

PROVIDER: S-EPMC6177421 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature