Superiority of SpiroZin2 Versus FluoZin-3 for monitoring vesicular Zn²⁺ allows tracking of lysosomal Zn²⁺ pools.

ABSTRACT: Small-molecule fluorescent probes are powerful and ubiquitous tools for measuring the concentration and distribution of analytes in living cells. However, accurate characterization of these analytes requires rigorous evaluation of cell-to-cell heterogeneity in fluorescence intensities and intracellular distribution of probes. In this study, we perform a parallel and systematic comparison of two small-molecule fluorescent vesicular Zn²⁺ probes, FluoZin-3 AM and SpiroZin2, to evaluate each probe for measurement of vesicular Zn²⁺ pools. Our results reveal that SpiroZin2 is a specific lysosomal vesicular Zn²⁺ probe and affords uniform measurement of resting Zn²⁺ levels at the single cell level with proper calibration. In contrast, FluoZin-3 AM produc

SUBMITTER: Han Y 

PROVIDER: S-EPMC6177427 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature