An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria.

ABSTRACT: Flavin-binding fluorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efficacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (¹O₂) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate ¹O₂, all of the other tested flavoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable differences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can

SUBMITTER: Endres S 

PROVIDER: S-EPMC6177443 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature