P25 of the dynactin complex plays a dual role in cargo binding and dynactin regulation.

ABSTRACT: Cytoplasmic dynein binds its cargoes via the dynactin complex and cargo adapters, and the dynactin pointed-end protein p25 is required for dynein-dynactin binding to the early endosomal dynein adapter HookA (Hook in the fungus Aspergillus nidulans). However, it is unclear whether the HookA-dynein-dynactin interaction requires p27, another pointed-end protein forming heterodimers with p25 within vertebrate dynactin. Here, live-cell imaging and biochemical pulldown experiments revealed that although p27 is a component of the dynactin complex in A. nidulans, it is dispensable for dynein-dynactin to interact with ΔC-HookA (cytosolic HookA lacking its early endosome-binding C terminus) and is not critical for dynein-mediated early endosome transport. Using mutagenesis, imaging, an

SUBMITTER: Qiu R 

PROVIDER: S-EPMC6177580 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature