Glycoside hydrolase family 18 and 20 enzymes are novel targets of the traditional medicine berberine.

ABSTRACT: Berberine is a traditional medicine that has multiple medicinal and agricultural applications. However, little is known about whether berberine can be a bioactive molecule toward carbohydrate-active enzymes, which play numerous vital roles in the life process. In this study, berberine and its analogs were discovered to be competitive inhibitors of glycoside hydrolase family 20 β-N-acetyl-d-hexosaminidase (GH20 Hex) and GH18 chitinase from both humans and the insect pest Ostrinia furnacalis Berberine and its analog SYSU-1 inhibit insect GH20 Hex from O. furnacalis (OfHex1), with K_i values of 12 and 8.5 μm, respectively. Co-crystallization of berberine and its analog SYSU-1 in complex with OfHex1 revealed that the positively charged conjug

SUBMITTER: Duan Y 

PROVIDER: S-EPMC6177593 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature