Deletion of mitochondrial calcium uniporter incompletely inhibits calcium uptake and induction of the permeability transition pore in brain mitochondria.

ABSTRACT: Ca²⁺ influx into mitochondria is mediated by the mitochondrial calcium uniporter (MCU), whose identity was recently revealed as a 40-kDa protein that along with other proteins forms the mitochondrial Ca²⁺ uptake machinery. The MCU is a Ca²⁺-conducting channel spanning the inner mitochondrial membrane. Here, deletion of the MCU completely inhibited Ca²⁺ uptake in liver, heart, and skeletal muscle mitochondria. However, in brain nonsynaptic and synaptic mitochondria from neuronal somata/glial cells and nerve terminals, respectively, the MCU deletion slowed, but did not completely block, Ca²⁺ uptake. Under resting conditions, brain MCU-KO mitochondria remained polarized, and in brain MCU-KO mitochondria, the electrophoretic Ca²⁺

SUBMITTER: Hamilton J 

PROVIDER: S-EPMC6177608 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature