Humanized GPIbα-von Willebrand factor interaction in the mouse.

ABSTRACT: The interaction of platelet glycoprotein Ibα (GPIbα) with von Willebrand factor (VWF) initiates hemostasis after vascular injury and also contributes to pathological thrombosis. GPIbα binding to the VWF A1 domain (VWFA1) is a target for antithrombotic intervention, but attempts to develop pharmacologic inhibitors have been hindered by the lack of animal models because of the species specificity of the interaction. To address this problem, we generated a knockin mouse with Vwf exon 28-encoding domains A1 and A2 replaced by the human homolog (VWF^h28). VWF^h28 mice (M1HA) were crossbred with a transgenic mouse strain expressing human GPIbα on platelets (mGPIbα^null;hGPIbα^Tg; H1MA) to generate a new strain (H1HA) with humanized GPIbα-VWFA1 bindi

SUBMITTER: Kanaji S 

PROVIDER: S-EPMC6177644 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature