A novel detection method of cleaved plasma high-molecular-weight kininogen reveals its correlation with Alzheimer's pathology and cognitive impairment.

ABSTRACT:

Introduction

Accumulation of β-amyloid is a pathological hallmark of Alzheimer's disease (AD). β-Amyloid activates the plasma contact system leading to kallikrein-mediated cleavage of intact high-molecular-weight kininogen (HKi) to cleaved high-molecular-weight kininogen (HKc). Increased HKi cleavage is observed in plasma of AD patients and mouse models by Western blot. For potential diagnostic purposes, a more quantitative method that can measure HKc levels in plasma with high sensitivity and specificity is needed.

Methods

HKi/c, HKi, and HKc monoclonal antibodies were screened from hybridomas using direct ELISA with a fluorescent substrate.

Results

We generated monoclonal antibodies recognizing HKi or HKc specifically and developed sandwich ELISAs that can quantitat

SUBMITTER: Yamamoto-Imoto H 

PROVIDER: S-EPMC6178129 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature