Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells.

ABSTRACT: Understanding the cellular properties controlling neural stem and progenitor cell (NSPC) fate choice will improve their therapeutic potential. The electrophysiological measure whole-cell membrane capacitance reflects fate bias in the neural lineage but the cellular properties underlying membrane capacitance are poorly understood. We tested the hypothesis that cell surface carbohydrates contribute to NSPC membrane capacitance and fate. We found NSPCs differing in fate potential express distinct patterns of glycosylation enzymes. Screening several glycosylation pathways revealed that the one forming highly branched N-glycans differs between neurogenic and astrogenic populations of cells in vitro and in vivo. Enhancing highly branched N-glycans on NSPCs significantly increases membrane capaci

SUBMITTER: Yale AR 

PROVIDER: S-EPMC6178213 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature