Evaluation of Spectral Counting for Relative Quantitation of Proteoforms in Top-Down Proteomics.

ABSTRACT: Spectral counting is a straightforward label-free quantitation strategy used in bottom-up proteomics workflows. The application of spectral counting in label-free top-down proteomics workflows can be similarly straightforward but has not been applied as widely as quantitation by chromatographic peak areas or peak intensities. In this study, we evaluate spectral counting for quantitative comparisons in label-free top-down proteomics workflows by comparison with chromatographic peak areas and intensities. We tested these quantitation approaches by spiking standard proteins into a complex protein background and comparing relative quantitation by spectral counts with normalized chromatographic peak areas and peak intensities from deconvoluted extracted ion chromatograms of the spiked proteins.

SUBMITTER: Geis-Asteggiante L 

PROVIDER: S-EPMC6178225 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature