Analysis of procoagulant phosphatidylserine-exposing platelets by imaging flow cytometry.

ABSTRACT:

Background

Upon platelet activation, a subpopulation of procoagulant platelets is formed, characterized by the exposure of the anionic aminophospholipid phosphatidylserine (PS) on the surface membrane.

Objective

To evaluate procoagulant PS-exposing platelets by imaging flow cytometry.

Methods

Platelet ultrastructure was examined by transmission electron microscopy, and a comprehensive analysis of procoagulant platelets was performed using imaging flow cytometry; platelets were fluorescently labeled for the markers glycoprotein (GP)IX, activated integrin αIIbβ3, CD62P, and PS exposure.

Results

A subpopulation of platelets stimulated in suspension by the physiological agonists thrombin+collagen, and all platelets stimulated by the calcium ionophore A23187, had a

SUBMITTER: Reddy EC 

PROVIDER: S-EPMC6178738 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature