Proteomics investigation of the changes in serum proteins after high- and low-flux hemodialysis.

ABSTRACT:

Purpose

This study aimed to use proteomics methods to investigate the changes in serum protein levels after high- and low-flux hemodialysis (HD).

Methods

Before and after HD, serum samples were obtained from two selected patients who were treated with a Polyflux 140 H high-flux dialyzer and a Polyflux 14 L low-flux dialyzer during two continuous therapy sessions. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was performed to identify the proteins.

Results

A total of 212 and 203 serum proteins were identified after high-flux and low-flux HD, respectively. After high-flux HD, 21 proteins increased, and 132 proteins decreased. After low-flux HD, 87 proteins increased, and 45 proteins decreased. High-flux HD led to a significantly greater reduction in protein

SUBMITTER: Han S 

PROVIDER: S-EPMC6179064 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature