Effectiveness of PCR primers for the detection of occult hepatitis B virus infection in Mexican patients.

ABSTRACT:

Background

Occult hepatitis B infection (OBI) is defined as the presence of hepatitis B virus (HVB) DNA in the liver of HBsAg negative individuals with or without detectable viral DNA in serum. OBI is a diagnostic challenge as it is characterized by a very low viral load, intermittently detectable through time. Individuals with OBI can develop chronic hepatic disease, including liver cirrhosis and hepatocellular carcinoma. The aim of this work was to produce tools to improve OBI detection of the HVB genotypes prevalent in Mexico.

Methods

We designed and tested primers to detect OBI in serum samples by nested and real-time PCR. Conserved sites in the viral genome were determined by alignment of the most frequent HBV genotypes in Mexico (H, G/H, F and D) and primers spanning t

SUBMITTER: Sosa-Jurado F 

PROVIDER: S-EPMC6179258 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature