Blind sparse inpainting reveals cytoskeletal filaments with sub-Nyquist localization.

ABSTRACT: Single-molecule localization microscopy (SMLM), such as stochastic optical reconstruction microscopy and (fluorescence) photoactivated localization microscopy, has enabled superresolution microscopy beyond the diffraction limit. However, the temporal resolution of SMLM is limited by the time needed to acquire sufficient sparse single-molecule activation events to successfully construct a superresolution image. Here, a novel fast SMLM technique is developed to achieve superresolution imaging within a much shortened duration. This technique does not require a faster switching rate or a higher activation density, which may cause signal degradation or photodamage/bleaching, but relies on computational algorithms to reconstruct a high-density superresolution image from a low-density one using t

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6179357 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature