Estradiol Drives the Anorexigenic Activity of Proopiomelanocortin Neurons in Female Mice.

ABSTRACT: Energy balance is regulated by anorexigenic proopiomelanocortin (POMC) and orexigenic neuropeptide Y/agouti-related peptide (NPY/AgRP) neurons of the hypothalamic arcuate nucleus. POMC neurons make extensive projections and are thought to release both amino acid and peptide neurotransmitters. However, whether they communicate directly with NPY/AgRP neurons is debated. Initially, using single-cell RT-PCR, we determined that mouse POMC^eGFP neurons express Slc17a6 (Vglut2) and Slc18a2 (Vmat2), but not Slc31a1 (Vgat) mRNA, suggesting glutamate and non-canonical GABA release. Quantitative (q)RT-PCR of POMC^eGFP cells revealed that Vglut2 and Vmat2 expression was significantly increased in E2- versus oil-treated, ovarie

SUBMITTER: Stincic TL 

PROVIDER: S-EPMC6179576 | biostudies-literature | 2018 Jul-Aug

REPOSITORIES: biostudies-literature