Divergent wiring of repressive and active chromatin interactions between mouse embryonic and trophoblast lineages.

ABSTRACT: The establishment of the embryonic and trophoblast lineages is a developmental decision underpinned by dramatic differences in the epigenetic landscape of the two compartments. However, it remains unknown how epigenetic information and transcription factor networks map to the 3D arrangement of the genome, which in turn may mediate transcriptional divergence between the two cell lineages. Here, we perform promoter capture Hi-C experiments in mouse trophoblast (TSC) and embryonic (ESC) stem cells to understand how chromatin conformation relates to cell-specific transcriptional programmes. We find that key TSC genes that are kept repressed in ESCs exhibit interactions between H3K27me3-marked regions in ESCs that depend on Polycomb repressive complex 1. Interactions that are prominent in TSCs

SUBMITTER: Schoenfelder S 

PROVIDER: S-EPMC6180096 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature