Biocatalyst-artificial metalloenzyme cascade based on alcohol dehydrogenase.

ABSTRACT: Chemo-enzymatic cascades of enzymes with transition metal catalysts can offer efficient synthetic strategies, but their development is challenging due to the incompatibility between proteins and transition metal complexes. Rhodium catalysts can be combined with alcohol dehydrogenases to regenerate nicotinamide cofactors using formate as the hydride donor. However, their use is limited, due to binding of the metals to residues on the enzyme surface, leading to mutual enzyme and catalyst inactivation. In this work, we replaced the zinc from Thermoanaerobacter brockii alcohol dehydrogenase (TbADH) with Rh(iii) catalysts possessing nitrogen donor ligands, by covalent conjugation to the active site cysteine, to create artificial metalloenzymes for NADP⁺ reduction. TbADH was us

SUBMITTER: Morra S 

PROVIDER: S-EPMC6180310 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature