R-spodin2 enhances canonical Wnt signaling to maintain the stemness of glioblastoma cells.

ABSTRACT:

Background

As newly identified Wnt enhancer, R-spondin gene family members have been linked to various cancers; however, their role in isocitrate dehydrogenase-wildtype subtype of human glioblastoma (GBM) cells remains unknown.

Methods

Human U87 and U251 cell lines were used to perform the experiments. GBM stem-like cells were enriched in stem cell growth media and induced to differentiate using retinoid acid or growth factor deprivation. Wnt^high and Wnt^low subpopulations were isolated and evaluated by MTS, sphere formation, transwell migration and xenograft formation assays.

Results

R-spondin 2 but not R-spondin 3 potentiates Wnt/β-catenin signaling in GBM cell lines. While R-spondin 2 does not affect cell growth, it induces the expression of pl

SUBMITTER: Liu S 

PROVIDER: S-EPMC6180579 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature