Serially transplantable mammary epithelial cells express the Thy-1 antigen.

ABSTRACT:

Background

Recent studies in murine mammary tissue have identified functionally distinct cell populations that may be isolated by surface phenotype or lineage tracing. Previous groups have shown that CD24^medCD49f^high cells enriched for long-lived mammary epithelial cells can be serially transplanted.

Methods

Flow cytometry-based enrichment of distinct phenotypic populations was assessed for their gene expression profiles and functional proliferative attributes in vitro and in vivo.

Results

Here, we show Thy-1 is differentially expressed in the CD24^medCD49f^high population, which allowed us to discern two functionally different populations. The Thy-1⁺CD24^medCD49f^high phenotype contained the maj

SUBMITTER: Lobo NA 

PROVIDER: S-EPMC6180607 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature