Consistent and reproducible cultures of large-scale 3D mammary epithelial structures using an accessible bioprinting platform.

ABSTRACT:

Background

Standard three-dimensional (3D) in vitro culture techniques, such as those used for mammary epithelial cells, rely on random distribution of cells within hydrogels. Although these systems offer advantages over traditional 2D models, limitations persist owing to the lack of control over cellular placement within the hydrogel. This results in experimental inconsistencies and random organoid morphology. Robust, high-throughput experimentation requires greater standardization of 3D epithelial culture techniques.

Methods

Here, we detail the use of a 3D bioprinting platform as an investigative tool to control the 3D formation of organoids through the "self-assembly" of human mammary epithelial cells. Experimental bioprinting procedures were optimized to enable the forma

SUBMITTER: Reid JA 

PROVIDER: S-EPMC6180647 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature