The DNA methylation landscape of glioblastoma disease progression shows extensive heterogeneity in time and space.

ABSTRACT: Glioblastoma is characterized by widespread genetic and transcriptional heterogeneity, yet little is known about the role of the epigenome in glioblastoma disease progression. Here, we present genome-scale maps of DNA methylation in matched primary and recurring glioblastoma tumors, using data from a highly annotated clinical cohort that was selected through a national patient registry. We demonstrate the feasibility of DNA methylation mapping in a large set of routinely collected FFPE samples, and we validate bisulfite sequencing as a multipurpose assay that allowed us to infer a range of different genetic, epigenetic, and transcriptional characteristics of the profiled tumor samples. On the basis of these data, we identified subtle differences between primary and recurring tumors, links

SUBMITTER: Klughammer J 

PROVIDER: S-EPMC6181207 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature