Hydrogen sulfide increases glutathione biosynthesis, and glucose uptake and utilisation in C₂C₁₂ mouse myotubes.

ABSTRACT: Diabetic patients have lower blood concentrations of hydrogen sulfide (H₂S), L-cysteine (LC), and glutathione (GSH). Using C₂C₁₂ mouse myotubes as a model, this study investigates the hypothesis that the beneficial effects of LC supplementation are mediated by upregulation of the H₂S status under diabetic conditions. Results show that exogenous administration of sodium hydrosulfide (NaHS, 10 or 20 µM; 6 hours), a H₂S donor, significantly (p < .05) upregulates the gene expression of cystathionine-γ-lyase (CSE), LC transporter (Slc7a11/xCT), and the genes involved in GSH biosynthesis. Additionally, it reduces homocysteine (HCys), reactive oxygen species (ROS) production, and enhances cellular LC, H₂S, and glucose uptake and ut

SUBMITTER: Parsanathan R 

PROVIDER: S-EPMC6181233 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature