Structure-Function Changes of the Porcine Distal Outflow Tract in Response to Nitric Oxide.

ABSTRACT:

Purpose

To correlate outflow function and outflow tract vessel diameter changes induced by nitric oxide (NO).

Methods

In a porcine anterior segment perfusion model, the effects of a nitric oxide donor (100 μM DETA-NO) on outflow facility were compared with controls (n = 8 per group) with trabecular meshwork (TM) and after circumferential ab interno trabeculectomy (AIT). Outflow structures were assessed with spectral-domain optical coherence tomography (SD-OCT) before and after NO, or an NO synthase inhibitor (100 μM L-NAME) and the vasoconstrictor, endothelin-1 (100 pg/mL ET-1). Scans were processed with a custom macroscript and aligned for automated reslicing and quantification of cross-sectional outflow tract areas (CSA).

Results

The facility increased after DETA-NO

SUBMITTER: Waxman S 

PROVIDER: S-EPMC6181305 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature